Blood Coagulation – Pubmed

Acute pancreatitis (AP) is a common and rapidly progressing disease of the digestive system. The occurrence of coagulation dysfunction is closely related to the severity of AP. Therefore, this study aims to explore the correlation and mechanism of early clinical coagulation function indicators in AP patients with the severity of the condition. Between January 2021 and May 2023, 60 cases of AP patients were divided into 2 groups: mild AP and severe AP. Coagulation parameters, including...

Cornus plants have found extensive applications in food and exhibit a versatile and complex pharmacological potential due to their broad spectrum of bioactive compounds. The aim of our study was to investigate and compare the composition of phenolic compounds and the inhibitory activity of leaf extracts of three species of the genus Cornus: C. alba, C. sanguinea, and C. sericea on pancreatic lipase, α-amylase, and blood coagulation in vitro. A total of 42 compounds classified as terpenoids and...

INTRODUCTION: Bonobos (Pan paniscus) are endangered and uncommon in managed care. Prothrombotic and hypofibrinolytic states are encountered with cardiovascular disease, ischemic stroke, and neoplasia.

Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease with excessive accumulation of extracellular matrix (ECM) components, which leads to disrupted lung architecture and respiratory failure. Interleukin (IL)-17 signaling induces fibrosis through inflammation; however, specific roles of its receptors IL-1RA and IL-17RC in fibrinolysis and ECM remodeling remain unclear. We hypothesized that inhibition of either IL-17RA or IL-17RC could reduce fibrosis by regulating MMP...

Sepsis-associated acute respiratory distress syndrome (ARDS) is characterized by excessive inflammation and dysregulated intra-alveolar coagulation, leading to fibrin deposition and impaired alveolar function. The regulatory role of vitamin D-vitamin D receptor (VDR) signaling in epithelial coagulation responses remains incompletely defined. We investigated the role of vitamin D-VDR signaling in regulating epithelial-driven intra-alveolar coagulation in sepsis-associated lung injury using...

CONCLUSION: In this comparative cross-sectional study, we found that patients with AN exhibit modest coagulation activation, characterized by elevated plasma TF and prothrombin fragment 1+2, alongside increased C1 inhibitor and reduced contact activation potential. This indicates that the balance between coagulation activators and inhibitors is generally maintained, but with an imbalance in the contact activation pathway that does not indicate a procoagulant state. The findings suggest a shift...

Vitamin K is essential for coagulation and has increasingly been recognized for its potential role in inflammatory processes. In patients with chronic viral hepatitis, alterations in coagulation parameters and persistent systemic inflammation are frequently observed. This study aimed to evaluate the association between vitamin K administration and changes in liver enzymes, inflammatory markers, and coagulation parameters in patients with chronic viral hepatitis. This retrospective observational...

Aging is strongly associated with an increased risk of morbidity and mortality from multiple diseases, including thromboembolic disorders. Endothelial dysfunction is considered a key contributor to age-related thrombus formation, although its underlying mechanisms remain incompletely understood. Cellular senescence is a fundamental driver of aging; however, the distinct functional roles of replicative (RS) and stress-induced premature senescence (SIPS) in cellular functions remain unclear. This...

CONCLUSION: These results demonstrate that storage in plasma at -80 °C effectively preserves platelet viability and functionality over a short period. This study highlights the potential for developing supplementary storage strategies as alternatives to conventional preservation methods, which could improve platelet availability in transfusion medicine.

CONCLUSION: Feminizing GAHT for 12 months had prothrombotic effects on the fibrin clot, whereas masculinizing GAHT had antithrombotic effects. The procoagulant effect was most evident with oral feminizing GAHT. This large cohort study provides important insights into the mechanisms linking GAHT to thrombotic risk.

The experimental aim of this study was to determine, in vitro, the effect of estrogen on brain endothelial cell oxidative stress, apoptotic susceptibility, and tissue-type plasminogen activator (t-PA) release. Human cerebral microvascular endothelial cells (hCMECs) were cultured and treated with 17β-estradiol (100 nM), the primary and most biologically active endogenous form of estrogen in humans, for 24 h. Intracellular reactive oxygen species production was significantly lower (∼15%; P > 0.01)...

Bleeding is an inherent risk in virtually all vascular and interventional radiology (VIR) procedures. Appropriately, all VIR preprocedural assessments should take into consideration a patient's coagulopathic state as a potential contraindication. Coagulopathic abnormalities are particularly common in critically ill patients, many of whom may require emergent, life-saving VIR interventions. As the landscape of VIR transitions toward an emphasis of VIR physicians serving as clinicians rather than...

CONCLUSIONS: In critically ill COVID-19 patients, anti-inflammatory treatment, particularly tocilizumab, is associated with reduced PAI-1 levels and accelerated clot lysis, indicating alleviated fibrinolysis.

CONCLUSIONS: This study identifies a novel SERPINB2-tPA regulatory axis in nasal epithelial cells. The convergent regulation by both Th1 and Th2 cytokines suggests that fibrinolytic dysfunction occurs across different CRSwNP inflammatory endotypes. These findings provide mechanistic insights into fibrin accumulation in nasal polyps and identify SERPINB2 as a potential therapeutic target for the prevention of polyp formation and recurrence.

The prevalence of depressive disorders continues to increase, representing a leading contributor to global disability and disease burden. Although antidepressants remain the cornerstone of treatment, their variable efficacy and adverse effects have drawn increasing attention. Vascular homeostasis is tightly regulated by the dynamic balance between coagulation and fibrinolysis, processes essential for maintaining physiological integrity. Emerging evidence indicates that antidepressants, beyond...

Rapid coagulation assessment is essential in critical care to enable timely correction of coagulopathy. Viscoelastic testing (VET) supports this goal but may be affected by mechanical stress during transport by pneumatic tube systems (PTS). As PTS are widely used to expedite sample delivery, evaluating the robustness of next-generation VET and platelet function assays under these conditions is crucial for reliable, time-sensitive diagnostics in intensive care. This study investigated the impact...

CONCLUSIONS: TXA efficacy is determined not only by dose but also by timing and the plasminogen availability in the clot microenvironment. These findings provide mechanistic insight into the failure of prophylactic TXA administration and highlight the importance of context in optimizing its clinical use.

CONCLUSIONS/SIGNIFICANCE: This study reveals key differences in the coagulant activity of BjV in human and rodent plasmas, underscoring the need to considering these interspecies disparities when using animal models in comparative envenomation research.

CONCLUSION: Integrating inflammatory and coagulation biomarkers improves surgical risk stratification in Crohn's disease and may help identify patients most likely to benefit from biologic therapy.

CONCLUSIONS: This study will provide evidence to support long-term safety monitoring of silver levels in women who have undergone silver clip sterilisation. It may increase awareness of potential health concerns, including inflammation and coagulation abnormalities. The findings may also contribute to the development of updated contraceptive guidelines for policymakers and women of childbearing age.

Hemorrhage is a leading cause of mortality in humans, responsible for approximately two million deaths annually worldwide. The contraction of blood clots is a robust process that occurs intravascularly to reduce the size of pathological thrombi. However, it is not clear if clot contraction contributes to hemostasis. Here we show that blood clots in laceration wounds contract, and the forces can decrease the size of wounds by up to 80%. Clots contracted after penetrating injuries in swine, as...

CONCLUSION: Higher exposure energy during HIFU treatment may induce alterations in coagulation function, resulting in a transient post-treatment hypercoagulable state.

CONCLUSION: These findings identify immune-coagulation dysregulation as a molecular link between PVTT, portal hypertension, and gastrointestinal bleeding risk in HCC and functionally validate OGFRL1 and WDR62 as biologically and therapeutically relevant targets.

Regenerative endodontic treatment (RET) is a biologically based approach aiming to restore the pulp-dentin complex in immature permanent teeth with necrotic pulps. In this procedure, a blood clot is induced within the canal to serve as a scaffold supporting stem cell migration, and its stability and regenerative success are closely linked to dentin surface morphology. This study focused exclusively on the quantitative assessment of surface roughness, without evaluating biological outcomes such...

CONCLUSIONS: CaCl(2)-crosslinked hydrogels offer a dual benefit of rapid hemostasis and enhanced wound healing, positioning them as valuable tools in wound care. Addressing current challenges through interdisciplinary research and clinical validation will maximize their therapeutic potential and that may contribute to improved standards in regenerative medicine.

Platelet activation is a key driver of in arterial thrombosis and myocardial infarction. Despite current anti-platelet therapies, mortality and morbidity from myocardial infarction remain high. Alternative anti-platelet therapies are needed that can reduce the risk of myocardial infarction without further increasing bleeding risk. Activated platelets rapidly diverge into distinct subpopulations. 'Procoagulant' platelets form through a mitochondria-dependent pathway. These procoagulant platelets...

CONCLUSION: Andexanet alfa was primarily prescribed for the reversal of FXa inhibitors in patients with intracerebral hemorrhage. We observed a lower incidence of thrombotic events than reported in clinical trials.

CONCLUSION: It was concluded that the formation of NET in the blood of patients with asthma is associated with impairment of clot retraction and fibrinolysis, which may contribute to the formation of unstable thrombi. This may partially explain the elevated risk of venous thromboembolism and pulmonary embolism in asthma.

Rapid removal of occluding blood clots, whether by intravenous thrombolysis or endovascular thrombectomy, can be life-saving. Yet recanalization is not always successful, and the determinants remain incompletely understood. Thrombi exhibit heterogeneous compositions, while clinical samples and animal models are costly, variable, and often lack reproducibility. To address these challenges, we present a blood clot analogue production system capable of generating tunable clot analogues in parallel...

Liver xenotransplantation has the potential to address the global shortage of donor organs; however, coagulation dysregulation remains a predominant barrier to long-term liver xenograft survival. In this study, orthotopic liver xenotransplantation was performed using a 10-gene-edited (GTKO/CMAHKO/β4GalNT2KO/hCD46/hCD55/hCD59/hTBM/hCD39/hEPCR/hCD47) porcine donor liver transplanted into a nonhuman primate recipient. Independent of graft function, which remained stable, an unexpected interruption...

Dengue virus (DENV) is the most prevalent arbovirus in the world, being transmitted to humans through the bite of infected Aedes mosquitoes. Symptomatic individuals may develop a haemorrhagic fever leading to plasma leakage, hypovolaemic shock and death. A key player in viral pathogenesis is the non-structural protein 1 (NS1), which is secreted by infected cells and disrupts platelet and endothelial barrier function, contributing to plasma extravasation. Importantly, dengue patients present...

Although unfractionated heparin (UFH) remains a vital agent for rapid anticoagulation, its reliance on animal-derived sources results in batch-to-batch variability and contamination risks, and its clinical application is further restricted by the possibility of heparin-induced thrombocytopenia. Dendritic polyglycerol sulfates were investigated as heparin analogues in 2004 due to their ability to mimic its charge, which is essential for its acting mechanism, and revealed an anticoagulant activity...

CONCLUSIONS: Weight-based PCC dosing yields inconsistent reductions in ROTEM-measured CT after cardiac surgery. EXTEM-CT is an unreliable predictor of severe postoperative bleeding, and PCC dosing should not be escalated solely based on EXTEM-CT prolongation.

Blood clots are pivotal for haemostasis and regeneration¹, but they are mechanically weak and form slowly², posing risks for life-threatening haemorrhage and limiting broader applications^(3-5). These limitations are attributed to complex coagulation cascades, abundant mechanically ineffective cells and little structural polymers. Strategies that strengthen polymer networks are inapplicable to these highly cellularized materials. Here we report a strategy that rapidly crosslinks red blood cells...

Objective:To investigate the preoperative coagulation characteristics and postoperative changes in patients with primary hyperparathyroidism（PHPT）. Methods:A retrospective analysis was conducted on the clinical data of 55 PHPT patients treated at Beijing Chaoyang Hospital, including preoperative and postoperatively（1 week） serum calcium, serum phosphorus, parathyroid hormone（PTH）, 25-hydroxyvitamin D, prothrombin time（PT）, activated partial thromboplastin time（APTT）, fibrinogen（FIB）, thrombin...

Innate immunity represents a foundational defense strategy across bilaterians, with phagocytosis and coagulation serving as its central effector mechanisms. However, it remains uncertain whether these mechanisms evolved from conservative ancestral regulatory modules or emerged through lineage-specific adaptations. This ambiguity currently impedes a deeper understanding of immune system evolution. We constructed a cross-phylum single-cell atlas of circulating immune cells from nine bilaterian...

CONCLUSIONS: Women with UI display unfavorably modified fibrin clot properties, especially those with negative pregnancy outcomes, which suggests the impact of fibrin clot characteristics on treatment results.

Psychological stress is a recognised, yet mechanistically unresolved, risk factor for cardiovascular disease (CVD) partly through its association with a hypercoagulable state. Free radical-mediated oxidative stress has been proposed as a key upstream driver of this haemostatic imbalance. In this randomised cross-over study we investigated whether acute psychological stress promotes systemic radical formation and prothrombotic alterations in clot microstructure in eight healthy males. The Trier...

CONCLUSION: NMRs possess the ability to bypass the pathologic clotting shifts that drive thrombotic events in humans, effectively decoupling chronologic aging from prothrombotic risk. By maintaining stable endothelial coagulation markers and an unchanged thrombin-forming capacity throughout their lifespan, NMRs appear naturally protected against age-dependent hypercoagulability.

CONCLUSIONS: Our findings support attenuation of platelet activation/coagulation by tocilizumab in patients with ST-segment-elevation MI. The marked rise in TF could be a hereto unrecognized side effects of tocilizumab, which need further investigation.

CONCLUSION: In vitro findings indicate a predictable concentration-response to abelacimab, with significant pharmacodynamics differences in young children. In vivo studies are required to confirm these findings, optimize dosing, and assess clinical implications in the pediatric population.

CONCLUSION: Through 18-48 h after delivery there is an increase in endogenous clotting potential compared to late pregnancy, centrifugation of this sedimentable material removes most of this endogenous activity.

CONCLUSIONS: We selected and validated sdAbs that specifically reverse the inhibitory effects of edoxaban, apixaban, and rivaroxaban, without procoagulant activity. These low-cost reversal agents work directly on plasma or whole blood without incubation or preanalytical steps. They enable rapid, reliable baseline coagulation testing in patients treated with these DOACs.

Mechanical heart valve replacement necessitates long-term warfarin anticoagulation to prevent thromboembolic complications, yet achieving adequate anticoagulation control remains challenging. This study evaluated whether mean platelet volume (MPV) could serve as a biomarker for anticoagulation quality in mechanical heart valve recipients. We conducted a retrospective longitudinal cohort analysis of 370 patients with mechanical heart valves receiving warfarin therapy. MPV and international...

Etamsylate is a classical hemostatic agent, used to control bleeding in various clinical settings. Yet, the precise mechanism of its action is unknown. Hereby, we evaluated the complex effect of etamsylate on platelet-dependent hemostasis, with the aim of supplementing the model of its mechanism of action. Fluorescence microscopy was used to study thrombus formation under flow, exposure of phosphatidylserine (PS), P-selectin, and PECAM-1 in adhered platelets. Flow cytometry was utilized to...

The biannual Fredrickson Lipid Research conference took place in person in Milwaukee, Wisconsin, from September 3-5, 2025. Each conference highlights the most advanced basic and translational research in lipids and lipoprotein metabolism. As with each Fredrickson Lipid Research conference, the overall theme is focused on "Lipid Metabolism, Lipoproteins, and Atherosclerosis," and this year included a special scientific session exploring the connections between Blood Clotting and Lipid Metabolism....

CONCLUSIONS: Anticoagulation monitoring during CPB in patients with APS remains heterogeneous and insufficiently standardized. The available evidence does not permit conclusions regarding comparative efficacy among monitoring strategies. Instead, this review highlights recurrent limitations of ACT-based monitoring and the heterogeneous use of adjunctive modalities in reported cases, providing a conceptual framework to inform future prospective investigations.

This study aimed to evaluate the appropriateness of terlipressin use and assess its effects on coagulation parameters in 240 hospitalized patients treated between January 2024 and January 2025 in a real-world, single-center retrospective cohort. Clinical appropriateness, encompassing diagnostic indication, initial dosing, and administration route, was rigorously evaluated against current clinical guidelines and the manufacturer's prescribing information. Key coagulation parameters - prothrombin...

Contrast-induced acute kidney injury (CI-AKI) is a major cause of hospital-acquired AKI, but its molecular pathogenesis remains incompletely understood. In this study, we used quantitative 4D proteomics, integrating ion mass-to-charge ratio (m/z), retention time, ion intensity, and ion mobility, to profile renal tissues from a novel rat CI-AKI model based on renal venous congestion and contrast exposure, with sham-operated rats as controls. Differentially expressed proteins were identified and...

To develop a high-efficiency and low-cost natural composite hemostatic material, this study has fabricated a series of Crinis Carbonisatus-Sepiolite (Cri-Sep) composites via solid-state reaction, leveraging the excellent hemostatic activity of Cri and the high adsorption capacity of Sep. The synergistic hemostatic mechanism and performance of these composites have been systematically investigated through experimental analysis. The fibrous microstructure of Sep, combined with the porous...